| Species Name | Status | See Also |
| Bat, gray | E | MO |
| Bat, Indiana | E | IN |
| Squirrel, Carolina northern flying | E | |
| Wolf, red | E | NC |
| Eagle, bald | E | IN, KS, LA, ME, MN, MO, MT, OH, WI, WY |
| Falcon, American peregrine | E | AK, CA, MN, MT, NY, OH, PA, VT, WI |
| Falcon, Arctic peregrine | T | AK |
| Tern, least (interior population) | E | IN, CA, ND, SD |
| Woodpecker, red-cockaded | E | AR, SC |
| Chub, slender | T | |
| Chub, spotfin | T | |
| Dace, blackside | T | |
| Darter, boulder (=Elk River) | E | |
| Darter, slackwater | T | |
| Darter, snail | T | |
| Logperch, Conasauga | E | |
| Madtom, smoky | E | |
| Madtom, yellowfin | T | |
| Shiner, blue | T | |
| Sturgeon, pallid | E | MO, ND |
| Snail, painted snake coiled forest | T | |
| Fanshell | E | |
| Mussel, cumberland pigtoe | E | |
| Mussel, ring pink (=golf stick pearly) | E | |
| Pearly mussel, Alabama lamp | E | |
| Pearly mussel, Appalachian monkeyface | E | |
| Pearly mussel, birdwing | E | |
| Pearly mussel, cracking | E | |
| Pearly mussel, Cumberland bean | E | |
| Pearly mussel, Cumberland monkeyface | E | |
| Pearly mussel, dromedary | E | |
| Pearly mussel, green-blossom | E | |
| Pearly mussel, little-wing | E | KY, NC, VA, |
| Pearly mussel (=pimple back), orange-footed | E | |
| Pearly mussel, pale lilliput | E | |
| Pearly mussel, pink mucket | E | |
| Pearly mussel, purple cat's paw | E | |
| Pearly mussel, tubercled-blossom | E | |
| Pearly mussel, turgid-blossom | E | |
| Pearly mussel, white wartyback | E | |
| Pearly mussel, yellow-blossom | E | |
| Pigtoe, fine-rayed | E | |
| Pigtoe, rough | E | |
| Pigtoe, shiny | E | |
| Riffle shell, tan | E | |
| Crayfish, Nashville | E | |
| Aster, Ruth's golden | E | |
| Avens, spreading | E | |
| Bluet, Roan Mountain | E | |
| Coneflower, Tennessee purple | E | |
| Fern, American hart's-tongue | T | |
| Goldenrod, Blue Ridge | T | |
| Grass, Tennessee yellow-eyed | E | |
| Ground-plum, Guthrie's | E | |
| Pitcher-plant, green | E | AL |
| Potato-bean, Price's | T | |
| Prairie-clover, leafy | E | |
| Rosemary, Cumberland | T | |
| Sandwort, Cumberland | E | |
| Skullcap, large-flowered | E | |
| Spiraea, Virginia | T | |
