USGS - science for a changing world

Northern Prairie Wildlife Research Center

  Home About NPWRC Our Science Staff Employment Contacts Common Questions About the Site

Caterpillars of Eastern Forests

Preserving Specimens

Properly preserved specimens provide the most definitive vouchers for identification and taxonomic study. The authoritative descriptions found in monographs and other comprehensive works virtually depend on the availability of preserved material. Immature stages for about 10% of the moths in Covell's (1984) Peterson Field Guide to Eastern Moths are still unknown, and hence by recording life history information, preserving, and photographing immature stages, even the casual observer or backyard naturalist has the opportunity to make important entomological contributions.

Preferred larval fixatives based on alcohol, formaldehyde, and glacial acetic acid are discussed in Stehr (1987). As an alternative, placing larvae into water that has been brought to a rolling boil and removed from the heat will yield excellent specimens. Once the larva is fully distended (after a few seconds to a minute in hot water) it should be transferred to a vial of 70% ethanol. Caterpillars killed simply by immersion in 70% ethanol frequently turn dark, presumably because microbial activity in the gut continues for some period of time. Regardless of the fluid method chosen, the gut contents often leak out over the first few days—it is often desirable to change the ethanol prior to archival storage. Some success may be obtained by placing caterpillars in a self-defrosting freezer for several months. Although much of the color may be preserved in this way, the specimens end up shrunken and inflexible and are not especially useful as study specimens for taxonomic study.

Many caterpillar pigments are lost in fluids. Greens seem to be especially ephemeral in preserved material. The almost immediate color losses that accompany the fluid preservation of loopers can be most disconcerting. Photographs or other types of image-capturing techniques provide the best means of documenting the coloration of living individuals. Preserved collections should be labeled in full with locality, date of collection, and host and cross referenced to any photographs or reared material.

Previous Section -- Rearing Caterpillars
Return to Contents
Next Section -- Photographing Caterpillars

Accessibility FOIA Privacy Policies and Notices

Take Pride in America logo logo U.S. Department of the Interior | U.S. Geological Survey
Page Contact Information: Webmaster
Page Last Modified: Saturday, 02-Feb-2013 05:14:16 EST
Sioux Falls, SD [sdww54]